Journal: Photonix

Article Title: Real-time wide-field fluorescence lifetime imaging via single-snapshot acquisition for biomedical applications

doi: 10.1186/s43074-025-00216-0

Figure Lengend Snippet: Mesoscopic Fast 3D FLI using Single-snapshot RLD: a Schematic of the mesoscopic fluorescence lifetime imaging (FLI) system employing light-sheet illumination and dual detection channels for near-infrared (NIR) and shortwave infrared (SWIR) fluorescence emission. b Schematic representation of angular image acquisition, stacking, and translational correction for 3D volume reconstruction. (c-e) 3D visualization of AU565 tumor spheroids treated with Trastuzumab-Alexa Fluor 750 (TZM-AF750); the white scale bars in 3D and 2D slices (s6, s17, s24 and s32) are 500 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\mu$$\end{document} μ m. c Single-snapshot RLD for fast 3D fluorescence lifetime volume reconstruction, d NIR-I 3D fluorescence intensity distribution derived from intensity (INT) images. e NIR-II fluorescence intensity maps acquired with an InGaAs detector, providing low-scattering 3D intensity reconstruction. f Single-snapshot RLD as a function of depth. g Average photon counts across tumor spheroid slices as a function of depth. h Average number of active pixels corresponding to tumor spheroid regions per slice as a function of depth

Article Snippet: Human epidermal growth factor receptor 2 (HER2+) AU565 cells (ATCC CRL-2351; breast cancer) were cultured in complete RPMI 1640 cell culturing medium containing HEPES supplemented with fetal bovine serum and penicillin/streptomycin.

Techniques: Fluorescence, Imaging, Derivative Assay

Journal: Animals : an Open Access Journal from MDPI

Article Title: Baicalin Alleviates ADAM17/EGFR Axis-Induced Peritonitis in Weaned Piglets Infected by Glaesserella parasuis

doi: 10.3390/ani15162457

Figure Lengend Snippet: Baicalin inhibited the expression of ADAM17/EGFR axis-related proteins in porcine peritoneal mesothelial cells infected with Glaesserella parasuis . Baicalin reduced the upregulation of ADAM17, p-EGFR/EGFR, and p-ERK/ERK. GPS stands for Glaesserella parasuis ; BA stands for baicalin; ADAM17 stands for disintegrin and metalloproteinase 17; EGFR stands for epidermal growth factor receptor; p-EGFR stands for phosphorylated EGFR; ERK stands for extracellular signal-regulated kinase; p-ERK stands for phosphorylated ERK; GAPDH stands for glyceraldehyde-3-phosphate dehydrogenase. Significant differences are denoted as follows: # and ## represent p < 0.05 and p < 0.01, respectively, for the comparisons between the control group and the GPS infection group; * and ** represent p < 0.05 and p < 0.01, respectively, for the comparisons between the GPS infection group and the baicalin groups.

Article Snippet: The membranes were blocked with 5% nonfat milk or 5% bovine serum albumin (BSA) in Tris-buffered saline containing 0.1% Tween-20 (TBST), followed by incubation with the following specific primary antibodies at 4 °C overnight: ADAM17 antibody (Bioss, Beijing, China; 1:1000); epidermal growth factor receptor (EGFR) antibody (PTM BIO, Hangzhou, Zhejiang, China; 1:1000); extracellular signal-regulated kinase (ERK) antibody, phosphorylated ERK (p-ERK) antibody, and tumor necrosis factor alpha (TNF-α) antibody (Proteintech, Wuhan, Hubei, China; 1:1000); phosphorylated EGFR (p-EGFR) antibody, interleukin 1 beta (IL-1β) antibody, and interleukin 6 (IL-6) antibody (Abclonal, Wuhan, Hubei, China; 1:1000); glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody (Servicebio, Wuhan, Hubei, China; 1:5000).

Techniques: Expressing, Infection, Control

Journal: Animals : an Open Access Journal from MDPI

Article Title: Baicalin Alleviates ADAM17/EGFR Axis-Induced Peritonitis in Weaned Piglets Infected by Glaesserella parasuis

doi: 10.3390/ani15162457

Figure Lengend Snippet: Baicalin inhibited the expression of ADAM17/EGFR axis-related proteins and inflammatory proteins induced by ADAM17 overexpression in porcine peritoneal mesothelial cells. ( A ) Baicalin reduced the upregulation of ADAM17, p-EGFR/EGFR, and p-ERK/ERK. ( B ) Baicalin reduced the upregulation of TNF-α, IL-1β, and IL-6. OvADAM17 stands for overexpression of ADAM17; BA stands for baicalin; ADAM17 stands for disintegrin and metalloproteinase 17; EGFR stands for epidermal growth factor receptor; p-EGFR stands for phosphorylated EGFR; ERK stands for extracellular signal-regulated kinase; p-ERK stands for phosphorylated ERK; TNF-α stands for tumor necrosis factor alpha; IL-1β stands for interleukin-1 beta; IL-6 stands for interleukin-6; GAPDH stands for glyceraldehyde-3-phosphate dehydrogenase. Significant differences are denoted as follows: ## represents p < 0.01 for the comparison between the control group and the OvADAM17 group; * and ** represent p < 0.05 and p < 0.01, respectively, for the comparisons between the OvADAM17 group and the baicalin groups.

Article Snippet: The membranes were blocked with 5% nonfat milk or 5% bovine serum albumin (BSA) in Tris-buffered saline containing 0.1% Tween-20 (TBST), followed by incubation with the following specific primary antibodies at 4 °C overnight: ADAM17 antibody (Bioss, Beijing, China; 1:1000); epidermal growth factor receptor (EGFR) antibody (PTM BIO, Hangzhou, Zhejiang, China; 1:1000); extracellular signal-regulated kinase (ERK) antibody, phosphorylated ERK (p-ERK) antibody, and tumor necrosis factor alpha (TNF-α) antibody (Proteintech, Wuhan, Hubei, China; 1:1000); phosphorylated EGFR (p-EGFR) antibody, interleukin 1 beta (IL-1β) antibody, and interleukin 6 (IL-6) antibody (Abclonal, Wuhan, Hubei, China; 1:1000); glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody (Servicebio, Wuhan, Hubei, China; 1:5000).

Techniques: Expressing, Over Expression, Comparison, Control

Journal: Animals : an Open Access Journal from MDPI

Article Title: Baicalin Alleviates ADAM17/EGFR Axis-Induced Peritonitis in Weaned Piglets Infected by Glaesserella parasuis

doi: 10.3390/ani15162457

Figure Lengend Snippet: ADAM17 small interfering RNA (siRNA) inhibited the expression of ADAM17/EGFR axis-related proteins and inflammatory proteins in porcine peritoneal mesothelial cells infected by Glaesserella parasuis . ( A ) ADAM17 siRNA reduced the upregulation of ADAM17, p-EGFR/EGFR, and p-ERK/ERK. ( B ) ADAM17 siRNA reduced the upregulation of TNF-α, IL-1β, and IL-6. NC stands for negative control siRNA; siRNA stands for ADAM17 siRNA; GPS stands for Glaesserella parasuis ; ADAM17 stands for disintegrin and metalloproteinase 17; EGFR stands for epidermal growth factor receptor; p-EGFR stands for phosphorylated EGFR; ERK stands for extracellular signal-regulated kinase; p-ERK stands for phosphorylated ERK; TNF-α stands for tumor necrosis factor alpha; IL-1β stands for interleukin-1 beta; IL-6 stands for interleukin-6; GAPDH stands for glyceraldehyde-3-phosphate dehydrogenase. Significant differences are denoted as follows: # and ## represent p < 0.05 and p < 0.01, respectively, for the comparisons between the negative control siRNA group and the negative control siRNA plus GPS group; * and ** represent p < 0.05 and p < 0.01, respectively, for the comparisons between the corresponding negative control siRNA and ADAM17 siRNA groups, both in the presence and absence of GPS infection.

Article Snippet: The membranes were blocked with 5% nonfat milk or 5% bovine serum albumin (BSA) in Tris-buffered saline containing 0.1% Tween-20 (TBST), followed by incubation with the following specific primary antibodies at 4 °C overnight: ADAM17 antibody (Bioss, Beijing, China; 1:1000); epidermal growth factor receptor (EGFR) antibody (PTM BIO, Hangzhou, Zhejiang, China; 1:1000); extracellular signal-regulated kinase (ERK) antibody, phosphorylated ERK (p-ERK) antibody, and tumor necrosis factor alpha (TNF-α) antibody (Proteintech, Wuhan, Hubei, China; 1:1000); phosphorylated EGFR (p-EGFR) antibody, interleukin 1 beta (IL-1β) antibody, and interleukin 6 (IL-6) antibody (Abclonal, Wuhan, Hubei, China; 1:1000); glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody (Servicebio, Wuhan, Hubei, China; 1:5000).

Techniques: Small Interfering RNA, Expressing, Infection, Negative Control

Journal: Animals : an Open Access Journal from MDPI

Article Title: Baicalin Alleviates ADAM17/EGFR Axis-Induced Peritonitis in Weaned Piglets Infected by Glaesserella parasuis

doi: 10.3390/ani15162457

Figure Lengend Snippet: Baicalin alleviated inflammatory protein expression via the ADAM17/EGFR axis in the peritoneum of piglets infected with Glaesserella parasuis . ( A ) Baicalin reduced the upregulation of ADAM17, p-EGFR/EGFR, and p-ERK/ERK. ( B ) Baicalin reduced the upregulation of TNF-α, IL-1β, and IL-6. GPS stands for Glaesserella parasuis ; BA stands for baicalin; ADAM17 stands for disintegrin and metalloproteinase 17; EGFR stands for epidermal growth factor receptor; p-EGFR stands for phosphorylated EGFR; ERK stands for extracellular signal-regulated kinase; p-ERK stands for phosphorylated ERK; TNF-α stands for tumor necrosis factor alpha; IL-1β stands for interleukin-1 beta; IL-6 stands for interleukin-6; GAPDH stands for glyceraldehyde-3-phosphate dehydrogenase. Significant differences are denoted as follows: ## represents p < 0.01 for the comparison between the control group and the GPS challenge group; * and ** represent p < 0.05 and p < 0.01, respectively, for the comparisons between the GPS challenge group and the baicalin treatment groups.

Article Snippet: The membranes were blocked with 5% nonfat milk or 5% bovine serum albumin (BSA) in Tris-buffered saline containing 0.1% Tween-20 (TBST), followed by incubation with the following specific primary antibodies at 4 °C overnight: ADAM17 antibody (Bioss, Beijing, China; 1:1000); epidermal growth factor receptor (EGFR) antibody (PTM BIO, Hangzhou, Zhejiang, China; 1:1000); extracellular signal-regulated kinase (ERK) antibody, phosphorylated ERK (p-ERK) antibody, and tumor necrosis factor alpha (TNF-α) antibody (Proteintech, Wuhan, Hubei, China; 1:1000); phosphorylated EGFR (p-EGFR) antibody, interleukin 1 beta (IL-1β) antibody, and interleukin 6 (IL-6) antibody (Abclonal, Wuhan, Hubei, China; 1:1000); glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody (Servicebio, Wuhan, Hubei, China; 1:5000).

Techniques: Expressing, Infection, Comparison, Control

Journal: Journal of Nuclear Medicine

Article Title: Preirradiation of Spheroids with 225 Ac-Trastuzumab Improves Penetration of 225 Ac-Liposomes and MIRDcell Predictions of Responses to Drug Cocktails

doi: 10.2967/jnumed.124.269273

Figure Lengend Snippet: Penetration data in BT474 spheroids (200-µm radius) for Alexa Fluor 647-NHS-trastuzumab ( ), CFDA-SE liposomes ( ), and CFDA-SE liposomes after preirradiating spheroids with 6.5 kBq/mL 225 Ac-trastuzumab ( ). Shown are time-integrated average concentrations as function of distance from centers of spheroids (A) and correlated mean decays per cell using activity concentrations of 6.875 kBq/mL for all drugs (calculated with MIRDcell-Ã) (B). Distances beyond 200 µm represent medium.

Article Snippet: The spheroids comprised the human epidermal growth factor receptor 2–positive human BT474 breast cancer cell line, obtained from the American Type Culture Collection.

Techniques: Liposomes, Activity Assay

Journal: Journal of Nuclear Medicine

Article Title: Preirradiation of Spheroids with 225 Ac-Trastuzumab Improves Penetration of 225 Ac-Liposomes and MIRDcell Predictions of Responses to Drug Cocktails

doi: 10.2967/jnumed.124.269273

Figure Lengend Snippet: MIRDcell calculated mean decays per cell attributed to liposomes in BT474 spheroids based on experimental CFDA-SE liposome penetration data for (100% 225 Ac-liposome case from Howe et al. ) and (50% 225 Ac-liposome, 50% 225 Ac-trastuzumab case from newly retrieved data), and extrapolated/averaged (weighted) liposome penetration data for (70% 225 Ac-liposome, 30% 225 Ac-trastuzumab case) and (30% 225 Ac-liposome, 70% 225 Ac-trastuzumab case).

Article Snippet: The spheroids comprised the human epidermal growth factor receptor 2–positive human BT474 breast cancer cell line, obtained from the American Type Culture Collection.

Techniques: Liposomes

Journal: Journal of Nuclear Medicine

Article Title: Preirradiation of Spheroids with 225 Ac-Trastuzumab Improves Penetration of 225 Ac-Liposomes and MIRDcell Predictions of Responses to Drug Cocktails

doi: 10.2967/jnumed.124.269273

Figure Lengend Snippet: Comparison of BT474 experimental outgrowths taken from Howe et al. to MIRDcell (version 4.14)-predicted SFs as bar plot (A), linear regression plot (B), and Bland–Altman plot (C) (95% limit of agreement, −0.048 to 0.095). Predicted SFs shown were based on 225 Ac-trastuzumab in spheroids with no 225 Ac daughters present and 225 Ac-liposomes in spheroids with 225 Ac daughters present. 225 Ac-trastuzumab and 225 Ac-liposomes in medium both had 225 Ac daughters present. Bars on abscissa of panel A and legend for panels B and C are labeled to denote percentage of activity corresponding to 225 Ac-liposomes and 225 Ac-trastuzumab. For example, “L-100, A-0” is 100% 225 Ac-liposomes and 0% activity on trastuzumab antibodies. Error bars for experimental outgrowths correspond to SD. Final prediction uses penetration data for CFDA-SE liposomes after spheroids are exposed to 6.5 kBq/mL 225 Ac-trastuzumab for 50%/50% case; 70% and 30% 225 Ac-liposome cases use extrapolated/averaged penetration data shown in . For CFDA-SE liposomes, published penetration data that were used for old prediction did not involve pretreatment 225 Ac-trastuzumab. Only final predictions are shown in panels B and C.

Article Snippet: The spheroids comprised the human epidermal growth factor receptor 2–positive human BT474 breast cancer cell line, obtained from the American Type Culture Collection.

Techniques: Comparison, Liposomes, Labeling, Activity Assay

Journal: Journal of Nuclear Medicine

Article Title: Preirradiation of Spheroids with 225 Ac-Trastuzumab Improves Penetration of 225 Ac-Liposomes and MIRDcell Predictions of Responses to Drug Cocktails

doi: 10.2967/jnumed.124.269273

Figure Lengend Snippet: Mean absorbed dose due to 225 Ac-trastuzumab (antibodies) and 225 Ac-liposomes (liposomes) in BT474 spheroids and medium for cells at various radial depths within 200-µm-radius spheroids (top). Below each graph, corresponding MIRDcell 3D slice representation of spheroid is shown for equatorial slices of spheroids. Dark red dots in equatorial slices represent living cells, whereas pink dots represent dead cells.

Article Snippet: The spheroids comprised the human epidermal growth factor receptor 2–positive human BT474 breast cancer cell line, obtained from the American Type Culture Collection.

Techniques: Liposomes

Journal: Journal of Nuclear Medicine

Article Title: Preirradiation of Spheroids with 225 Ac-Trastuzumab Improves Penetration of 225 Ac-Liposomes and MIRDcell Predictions of Responses to Drug Cocktails

doi: 10.2967/jnumed.124.269273

Figure Lengend Snippet: MIRDcell (version 4.16) AI–predicted minimum number of decays necessary to achieve target SF < 0.0001 in BT474 spheroids treated with 225 Ac-trastuzumab (antibodies), 225 Ac-liposomes (liposomes), or combination thereof. 225 Ac daughters were assumed to migrate away from 225 Ac-trastuzumab and to remain near 225 Ac-liposomes within spheroid. MIRDcell AI was limited to maximum molar activity of 10 6 GBq/mol for both drugs and lower limit of 1.17 × 10 5 GBq/mol for antibodies only. No bar appearing for antibodies indicates that 225 Ac-trastuzumab alone could not achieve target SF. When optimized molar activities for combined therapy and liposome-only therapy were checked in Monte Carlo–based MIRDcell simulation, zero cell survivors were achieved for both therapies although liposomes required more total decays to achieve same result. “Liposome with preirradiation” indicates use of penetration profiles after preirradiation with 225 Ac-trastuzumab as in 50%/50% case, and “Liposome without preirradiation” indicates use of liposome penetration profile without preirradiation as in 100% 225 Ac-liposomes case.

Article Snippet: The spheroids comprised the human epidermal growth factor receptor 2–positive human BT474 breast cancer cell line, obtained from the American Type Culture Collection.

Techniques: Liposomes, Activity Assay